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Welcome to the website of the FEBS Advanced Course: “Functional imaging of cellular signals”.

Every year the van Leeuwenhoek Centre for Advanced Microscopy (LCAM) is organising a microspectroscopy course for young scientists, PhD students and postdocs, entitled “In the footsteps of van Leeuwenhoek”. This years course topic is focused on cellular signaling and is financed by FEBS.

 

About the course

The ability of cells to perceive and correctly respond to their environment is the basis of development, tissue repair, and immunity as well as normal tissue homeostasis. Errors in cell signaling are responsible for many diseases such as cancer, autoimmunity, and diabetes. By understanding cell signaling, diseases may be treated effectively and, theoretically, artificial tissues may be created. Microscopic techniques can be used to elucidate signal complex composition, organization and dynamics, as this reflects the functions of cells and their organelles.dubbelkernRGB1[1]

Microscopic techniques can be used to elucidate complex composition, organization and dynamics, as this reflects the functions of cells and their organelles. Because of the recent technological revolution in advanced light microscopy (i.e. super-resolution imaging, functional imaging of molecules) it is now possible to directly monitor the dynamics of (single) molecules in living cells. Several new microscopy techniques are ideally suited for studying molecular complexation but their application remains limited because most biologists have never been introduced to these technologies. This practical advanced course will be organized to provide students in this field the theoretical background and give hands-on experience of state-of-the-art microscopy techniques.

The practical sessions will cover several microscopy techniques to study intracellular signal molecules or biosensors, such as:

  • Confocal microscopy
  • Spinning disc and TIRF microscopy
  • Förster Resonance Energy Transfer (FRET)
  • Fluorescence lifetime imaging (FLIM)
  • Fluorescence Recovery After Photobleaching (FRAP)
  • Fluorescence Correlation Spectroscopy (FCS)